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Test Name:
Urinalysis, Routine, with High Volume Culture if Indicated, Catheterized - Less than 2 years


  • SBMF No:
    25174
  • Performance Lab Name:
    Hematology
  • Test Mnemonic:
    U PED/CHV
  • CPT Code:
    81001
    If indicated, add 87086
  • LOINC Code:
    19159-3; 50553-7; 20627-6; 53326-5; 50560-2; 57735-3; 53328-1; 50557-8; 50551-1; 53292-9; 5809-9; 11277-1; 50558-6; 60026-2
  • Test Includes:
    Macroscopic Exam:
         – Specific gravity
         – pH
         – Protein
         – Ketones
         – Glucose
         – Bilirubin
         – Blood
         – Nitrites
         – Leukocytes
    Microscopic Exam:
         – White Blood Cells (WBC)
         – Red Blood Cells (RBC)
         – Casts
         – Crystals
         – Epithelial cells
         – Bacteria
         – Yeast
    Colony Count
    Culture if Indicated
  • Also See:
    25275 – Urinalysis, Routine – Less than 2 years
    25274 – Urinalysis, Routine, with Culture (includes Colony Count) and Susceptibility if Indicated, Clean-Catch – Less than 2 years
    25374 – Urinalysis, Routine, with High Volume Culture if Indicated, Catheterized – Greater than 2 years
  • Spec Type:
    Urine, clean-catch
  • Spec Container:
    Sterile plastic container with tightly fitting lid for urinalysis
           –plus–
       if urine sample volume greater than 3.0 mL, Urine C&S Transport Kit for culture
         Note: if sample volume less than 3.0 mL, urine remaining in sterile plastic container
  • Alt Spec Type:
    Urine, catheterized
  • Pref Vol:
    Entire volume of timed urine specimens
  • Min Vol:
    At least 12.0 mL urine for urinalysis - plus - sufficient urine volume for culture
  • Fasting:
    No
  • Spec Collect:
    Standard procedure for clean-catch urine
    Standard procedure for catheterized urine
         – Note: Preferred specimen is early morning urine which often yields the highest bacterial counts from overnight incubation in bladder
  • Spec Process:

    Urinalysis Specimen:
         – If a portion of urine has been transferred to a urine C&S transport tube for possible culture (see below), the urine for routine urinalysis can be maintained room temperature for up to 2 hours
         – If portion of urine has NOT been transferred to urine C&S transport tube, urine for routine urinalysis and possible culture must be refrigerated within 20 minutes of collection
         – Urine specimens for routine urinalysis can remain refrigerated up to 12 hours

    Urine Culture Specimen - High Volume (3.0-5.0 mL):
         – Immediately transfer well-mixed urine from bottom of sterile container into gray-top transport tube with preservative from "Urine C&S Transport Kit"
         – Shake tube vigorously
         – Preserved urine culture specimens (transferred to transport tubes with preservative) can remain at room temperature, or refrigerated, for up to 24 hours

    Urine Culture Specimen - Low Volume (less than 3.0 mL):
         – If amount of specimen remaining after urinalysis is less than 3.0 mL (low volume), do not transfer to tube from "Urine C&S Transport Kit" (volume is insufficient)
         – Unpreserved urine culture specimens must be refrigerated within 20 minutes of collection
         – Unpreserved urine culture specimens can remain refrigerated for up to 24 hours

  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    2 hours room temperature (20-30°C)
    24 hours refrigerated (2-8°C)
  • Spec Reject:
    Urinalysis Specimen:
        – Not refrigerated within 2 hours of collection
        – Refrigerated within 2 hours of collection, but not received for testing within 12 hours of collection
    Urine Culture Specimen:
        – High Volume specimens transferred to “Urine C&S Transport Kit”, but not received for testing within 24 hours of collection
        – Low Volume specimens collected in sterile, plastic container, but not refrigerated within 20 minutes of collection
        – Low Volume specimens collected in sterile, plastic container and refrigerated within 20 minutes of collection, but not received for testing within 12 hours of collection
  • Spec Remarks:
    Note: Culture is indicated and will be performed if bacteria is present or WBC is 6 or greater
  • Methodology:
    Automated Reflectance Spectrophotometer and Flow Cytometry
  • Use:
    Diagnosis and treatment management of urinary tract infection (UTI)
  • Clinical Significance:
    Indicate urinary tract infection (UTI), injury to kidneys due to trauma, or other renal diseases
  • Reference Range:
    Specific gravity: 1.005-1.030
    pH: 5-8
    Protein: negative
    Bilirubin: negative
    Glucose: negative
    Ketones: negative
    Occult Blood: negative
    Crystals: Interpreted by physician (warm, freshly voided urine sediment from normal subjects almost never contains crystals, despite maximal concentration)
    Male
         – RBC: 0-4/ hpf (0-10/µL)
         – WBC: 0-5/ hpf (0-25/µL)
         – Epithelial Cells: 0-1/ hpf (0-4/µL)
         – Casts: 0-4 hyaline casts/ lpf (0-1.5/µL)
         – Bacteria: Negative = less than 545/ hpf (less than 3000/µL)
    Female
         – RBC: 0-4/hpf (0-10/µL)
         – WBC: 0-5/hpf (0-25/µL)
         – Epithelial Cells: 0-7/hpf (0-4/µL)
         – Casts: 0-4 hyaline casts/lpf (0-1.5/µL)
         – Bacteria: Negative = less than 545/hpf (less than 3000/µL)
  • Day Run:
    Sun-Sat (daily)
  • Time Run:
    As received
  • Time Reported:
    4 hours
  • Test Type:
    URINE

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