South Bend Medical Foundation - Your Community Laboratory

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Test Name:
Cell Count, Body Fluid

• SBMF No:
  25116
• Performance Lab Name:
  Hematology
• Test Mnemonic:
  BF CELL
• ABN:
  Not required
• CPT Code:
  89050
• LOINC Code:
  47938-6; 6824-7; 9335-1; 26466-3; 26455-6
• Also Known As:
  Body Fluid Cell Count
• Also See:
  25162 Body Fluid Differential
  23116 Body Fluid Total Protein
  25161 Body Fluid Crystals
• Spec Type:
  Body fluid
• Spec Container:
  Lavender top (EDTA) tube
• Pref Vol:
  3.0 mL
• Min Vol:
  0.5 mL
• Fasting:
  No
• Spec Collect:
  Collected by physician or nursing personnel
  Immediately after collection, gently invert lavender top tube several times to mix contents
  Document source of fluid on label and requisition
• Spec Process:
  Do not centrifuge
• Spec Store Transport:
  Refrigerated
• Spec Stability:
  4 hours room temperature (20-30°C)
  24 hours refrigerated (2-8°C)
• Spec Reject:
  Centrifuged or severely coagulated sample
• Methodology:
  Hemacytometer
• Use:
  To check fluids for presence of white blood cells (WBC) and red blood cells (RBC)
• Clinical Significance:
  

  Abnormal accumulation of serous fluid will occur when conditions that affect capillary hyrostatic pressure, colloidal pressure, or permeability and lymphatic drainage are present. Examples in each of these cases include congestive heart failure, hypoalbuminemia, pneumonia, and carcinoma.

  Serous fluids for laboratory examination are collected by needle aspiration from body cavities. These aspiration procedures could include thoracentesis (pleural), pericardiacentesis (pericardial), synovial (joint) fluid and paracentesis (peritoneal). Many pathologic conditions can cause a buildup (effusion) of serous fluids.

  Effusion of serous fluid can be placed into two general categories: transudates and exudates. A transudate disrupts the balance in the regulation of fluid filtration and reabsorption. An example is the changes in hydrostatic pressure created by congestive heart failure. Exudates are produced by conditions that directly involve the membranes of the particular cavity including infections and malignancies.

  Analysis of body fluid usually includes one or more of the following: cell count, differential, total protein, crystals, pH, specific gravity, glucose, albumin, amylase, sodium, potassium, chloride, LD, creatinine, urea nitrogen, uric acid, routine culture, and cytologic studies. Additional testing may include specialized microbiology cultures for AFB or fungus or testing for the presence of bilirubin. Only those tests that are ordered will be performed.

• Reference Range:
  

  Pleural fluid:
  WBC: less than 1,000 /cmm (less than 25% polys)
  RBC: 0

  Peritoneal fluid:
  WBC: less than 500 /cmm (less than 25% polys)
  RBC: less than 100,00 /cmm

  Pericardial fluid:
  WBC: less than 500 /cmm (less than 25% polys)
  RBC: 0

  Synovial fluid:
  WBC: less than 200 /cmm

  Physician must interpret results based upon origin of fluid and patient's clinical condition

• Additional Test Info:
  Samples with clots will be counted, if possible, and reported with the following disclaimer:
  "Cell count may not be accurate due to presence of clots"
• Day Run:
  Sun-Sat (daily)
• Time Run:
  As received
• Time Reported:
  4 hours
• Test Type:
  BODY FLUID