test directorySearch Results
Click here to search again.
Test Name:
Platelet Function Analysis, by PFA-100
- SBMF No:
25113 - Performance Lab Name:
Hem-Coag - Test Mnemonic:
PLT FUNC - ABN:
Not required - CPT Code:
85576 - LOINC Code:
24471-5 - Spec Type:
Whole blood - Spec Container:
Light blue top (3.2% buffered sodium citrate) tube - Pref Vol:
4.5 mL - Min Vol:
2.0 mL - Fasting:
No - Patient Prep:
Contact SBMF Client Services to arrange for a courier – to ensure that sample is received by SBMF within 4 hours of collection - Spec Collect:
Prior to collection, ensure that SBMF Client Services has been contacted for sample transport
Routine venipuncture using 21 gauge or larger needle
Collect blood directly into citrate tube
Discard sample and recollect if there is venous collapse or stoppage of blood flow during collection - Spec Process:
Do not centrifuge
Do not remove plasma from cells
Do not refrigerate or freeze - Spec Store Transport:
Room temperature
Immediately transport sample to testing site – must arrive in SBMF coagulation lab within 4 hours of collection
Do not transport via pneumatic tube system - Spec Stability:
4 hours room temperature (20-30°C) - Spec Reject:
Hemolyzed or non-citrate anticoagulated sample
Refrigerated or frozen sample
Sample greater than 4 hours old - Methodology:
Dade PFA-100 - determination of closure time (CT) - Use:
Detect platelet dysfunction induced by intrinsic platelet defects - Clinical Significance:
Platelet plug formation in the PFA-100 system is affected by low platelet counts and/or activity, inadequate plasma vonWillebrand factor status, and by low hematocrits because of the flow process. Patients with platelet dysfunction related to uremia, von Willebrand’s disease, and exposure to agents such as aspirin or other anti-platelet drugs will exhibit long closure times.
INTERPRETATION:
When COL/EPI is elevated and COL/ADP is normal, the elevated COL/EPI result is likely caused by aspirin or aspirin-like substance ingestion.
When COL/EPI is elevated and COL/ADP is elevated, the elevated COL/ADP indicates that aspirin or aspirin-like substance-induced prolonged closure time is unlikely. Evaluate the result in conjunction with clinical history and other laboratory findings. - Reference Range:
The following reference ranges have been established and confirmed using whole blood samples collected in 3.2% buffered sodium citrate:
COL/EPI: 88-198 seconds
COL/ADP: 58-121 seconds - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
COAGULATION
