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Test Name:
Dilute Russell Viper Venom Time

  • SBMF No:
  • Performance Lab Name:
    TMF Automated Laboratory / Hematology
  • Test Mnemonic:
  • CPT Code:
  • Also Known As:
  • Also See:
    25243 Lupus Anticoagulant Detection
  • Patient Prep:
    Collect prior to initiation of anticoagulant therapy.
  • Spec Collect:
    Light Blue top (3.2% buffered sodium citrate) tube(s)
    NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
    • Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
    • Fill Light Blue top tubes to stated tube volume
    • If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect Light Blue top coagulation study tube(s)
    • If multiple coagulation studies are requested, submit additional samples for each coagulation test
    • Immediately after collection, mix by gently inverting tube 3-4 times
  • Spec Process:
    Separate plasma from cells immediately
    • Promptly centrifuge 15 minutes
    • Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
    NOTE: Platelet/Buffy coat layer of sample must be avoided
    • Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
    • Use second plastic pipette to carefully transfer top portion of plasma leaving approximately 250 mcL discard in bottom of tube
    • Aliquot 2 mL PPP into clearly labeled plastic tubes, 1 mL per aliquot (Min: 1 mL)
    • Immediately freeze PPP samples
  • Spec Store Transport:
    CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
  • Spec Stability:
    Room temperature: 4 hours
    Refrigerated: Unacceptable
    Frozen: 2 weeks
  • Spec Reject:
    Clotted, hemolyzed, or non-frozen specimens. Citrate tube underfilled or overfilled.
  • Spec Remarks:
    Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results.
  • Methodology:
    Clot Detection
  • Use:
    Determine the presence of lupus-like anticoagulants (LA)
  • Additional Test Info:
    Lab Tests Online
  • Day Run:
  • Time Run:
    7:00 am
  • Time Reported:
    5:00 pm
  • Test Type: