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Test Name:
Dilute Russell Viper Venom Time
- SBMF No:
25050 - Performance Lab Name:
Hem-Coag - Test Mnemonic:
DRVVT - ABN:
Not required - CPT Code:
85613 - LOINC Code:
6303-2; 57838-5; 3281-3 - Also Known As:
dRVVT - Also See:
25243 Lupus Anticoagulant Detection - Spec Type:
Platelet-poor plasma (PPP) - Spec Container:
Light Blue top (3.2% buffered sodium citrate) tube - Pref Vol:
2.0 mL (Two aliquots, 1.0 mL each) - Min Vol:
1.0 mL - Fasting:
No - Patient Prep:
Collect prior to initiation of anticoagulant therapy - Spec Collect:
- Routine venipuncture
- • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
• Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
• Fill light blue top tube to stated tube volume
• If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
• If multiple coagulation studies are requested, submit additional samples for each coagulation test
• Immediately after collection, gently invert tube to mix
- Spec Process:
- Separate plasma from cells immediately
- • Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• NOTE: Buffy coat layer of sample must be avoided
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
• Immediately freeze PPP samples
- Spec Store Transport:
Frozen - Spec Stability:
4 hours room temperature (20-30°C)
2 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Citrate tube underfilled
Sample clotted or hemolyzed
Plasma not frozen within 4 hours of collection - Spec Remarks:
Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results - Methodology:
Clot Detection - Use:
Determine the presence of lupus-like anticoagulants (LA) - Clinical Significance:
Lupus-like anticoagulants (LA) are immunoglobulins that interfere with one or more of the in vitro phospholipid-dependent steps of coagulation, resulting in prolonged coagulation tests, especially APTT, dRVVT, and TTI. Circulating lupus-like anticoagulants are recognized as a major cause of both venous and arterial thromboembolic events. Often encountered in settings such as recurrent abortion, deep vein thrombosis, and arterial thrombosis, LA may initially present as a prolonged APTT screen.The antiphospholipid protein antibody family includes lupus-like anticoagulants and cardiolipin antibodies, either of which can cause the syndromes described above. Patients may present with lupus-like anticoagulants and cardiolipin antibodies, or with one or the other. The Circulating Anticoagulant Detection test (#25242) for lupus-like anticoagulants plus Cardiolipin IgG and IgM antibodies (#28102 and #28103) are recommended for patients suspected of having a hypercoagulapathy due to an antiphospholipid protein antibody syndrome. - Reference Range:
Normal: No lupus-like anticoagulants (LA) present
LA1: Less than or equal to 45 seconds
LA2: Less than or equal to 38 seconds - Day Run:
Mon-Fri - Time Run:
7:00 am - Time Reported:
5:00 pm - Test Type:
COAGULATION
