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Test Name:
CBC with Manual Differential

• SBMF No:
  25016
• Performance Lab Name:
  Hematology
• Test Mnemonic:
  CBCHMD
• ABN:
  May be required – Medical Necessity
• CPT Code:
  85007; 85027
• Test Includes:
  White Blood Cells (WBC)
  Red Blood Cells (RBC)
  Hemoglobin (HGB)
  Hematocrit (HCT)
  Mean Corpuscular Volume (MCV)
  Mean Corpuscular Hemoglobin (MCH)
  Mean Corpuscular Hemoglobin Concentration (MCHC)
  RBC Distribution Width (RDW)
  Platelets (PLT)
  Differential, Manual
• Also Known As:
  Complete Blood Count with Manual Differential
• Spec Type:
  Whole blood
• Spec Container:
  Lavender top (EDTA) tube
• Pref Vol:
  5.0 mL
• Min Vol:
  1.0 mL
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
  Tube must fill to one-half, or greater, of vacuum capacity
  Immediately after collection, gently invert tube to mix
• Spec Process:
  Do not centrifuge
  Do not remove plasma from cells
• Spec Store Transport:
  Refrigerated
  Optimally, specimen should be received for testing within 24 hours of collection time
• Spec Stability:
  8 hours room temperature (20-30°C)
  24 hours refrigerated (2-8°C)
  Frozen (-20°C): Unacceptable
• Spec Reject:
  Collection tube not filled at least ½ of vacuum capacity
  Clotted, hemolyzed, or centrifuged sample
• Methodology:
  Pulse Measurement technology, Hydrodynamic Focusing, RF (Radio-Frequency) detection, and DC (Direct Current) detection.
• Use:
  Evaluate anemia, leukemia, reaction to inflammation and infections, peripheral blood cellular characteristics, state of hydration and dehydration, polycythemia, hemolytic disease of the newborn, inherited disorders of red cells, white cells, and platelets; manage chemotherapy decisions; determine qualitative and quantitative variations in white cell numbers and morphology; morphology of red cells and platelet evaluation
• Clinical Significance:
  

  To rule out anemia, leukemia, inflammation, and infection
  To view peripheral blood cellular characteristics and quantitate red blood cells (RBC), white blood cells (WBC), and platelets.

  Also see:
  

• Reference Range:
  WBC: 4.0-11.0 K/μL
  RBC, Male: 3.9-5.9 M/μL
  RBC, Female: 3.7-5.4 M/μL
  Hgb, Male: 13.0-17.3 g/dL
  Hgb, Female: 11.4-15.4 g/dL
  Hct, Male: 39-53%
  Hct, Female: 35-47%
  MCV: 81-100 fl
  MCH, Male: 27-34 pg
  MCH, Female: 25-34 pg
  MCHC: 30-36 g/dL
  Platelets: 130-470 K/μL
  RDW: 12.2-15.2%
  Differential:
    Neutrophils: 37-77% (absolute result 1.67-8.47 x 10³/mL)
    Lymphocytes: 23-44% (absolute result 1.03-4.84x 10³/mL)
    Monocytes: 4-13% (absolute result 0.27-0.98 x 10³/mL)
    Eosinophils: 1-6% (absolute result 0.11-0.55 x 10³/mL)
    Basophils: 0-1% (absolute result 0.02-1.10 x 10³/mL)
• Critical Low Value:
  WBC: less than 2.5 K/μL
  Hgb: less than 8.0 g/dL
  Platelets: less than 30.0 K/μL
  Hct: less than 24.0%
• Critical High Value:
  WBC: greater than 30.0 K/μL
  Hgb: greater than 20.0 g/dL
  Platelets: greater than 1,000 K/μL
  Hct: greater than 60.0%
• Day Run:
  Sun-Sat (daily)
• Time Run:
  As received
• Time Reported:
  4 hours
• Test Type:
  HEMATOLOGY