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Test Name:
Hemoglobin Identification, by HPLC
- SBMF No:
23413 - Performance Lab Name:
Manual Lab - Test Mnemonic:
HGB EVAL - ABN:
Not required - CPT Code:
83021 - LOINC Code:
42245-1; 42246-9; 49322-1; 42247-7; 42244-4 - Also Known As:
Electrophoresis, Hemoglobin
Hemoglobin Electrophoresis
Hemoglobin Variant
Hemoglobinopathy Evaluation
Thalassemia Evaluation - Spec Type:
Whole blood - Spec Container:
Lavender top (EDTA) tube - Pref Vol:
2.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Do not centrifuge
Do not remove plasma from cells - Spec Store Transport:
Refrigerated - Spec Stability:
1 week refrigerated (2-8°C) - Methodology:
High Performance Liquid Chromatography (HPLC) - Use:
Diagnose hemoglobin abnormalities due to chain variant(s)
– such as sickle cell disease and trait, hemoglobin C disease and trait, etc.,
and abnormalities due to deficiencies or absence of chain formation, that is, all thalassemias - Reference Range:
Hgb A: 96.0-98.0%
Hgb A2: 2.0-3.4%
Hgb F:
- 0-3 weeks = 0.0-65.0%
- 3 weeks to 6 months = 0.0-2.0%
- 6 months to adult = 0.0-1.0% - Day Run:
Tue, Fri - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
CHEMISTRY