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Test Name:
Hemoglobin Identification, by HPLC


  • SBMF No:
    23413
  • Performance Lab Name:
    Manual Lab
  • Test Mnemonic:
    HGB EVAL
  • ABN:
    Not required
  • CPT Code:
    83021
  • LOINC Code:
    42245-1; 42246-9; 49322-1; 42247-7; 42244-4
  • Also Known As:
    Electrophoresis, Hemoglobin
    Hemoglobin Electrophoresis
    Hemoglobin Variant
    Hemoglobinopathy Evaluation
    Thalassemia Evaluation
  • Spec Type:
    Whole blood
  • Spec Container:
    Lavender top (EDTA) tube
  • Pref Vol:
    2.0 mL
  • Min Vol:
    0.5 mL
  • Fasting:
    No
  • Spec Collect:
    Routine venipuncture
  • Spec Process:
    Do not centrifuge
    Do not remove plasma from cells
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Methodology:
    High Performance Liquid Chromatography (HPLC)
  • Use:
    Diagnose hemoglobin abnormalities due to chain variant(s)
       – such as sickle cell disease and trait, hemoglobin C disease and trait, etc.,
          and abnormalities due to deficiencies or absence of chain formation, that is, all thalassemias
  • Reference Range:
    Hgb A: 96.0-98.0%
    Hgb A2: 2.0-3.4%
    Hgb F:
        - 0-3 weeks = 0.0-65.0%
        - 3 weeks to 6 months = 0.0-2.0%
        - 6 months to adult = 0.0-1.0%
  • Day Run:
    Tue, Fri
  • Time Run:
    8:00 am
  • Time Reported:
    5:00 pm
  • Test Type:
    CHEMISTRY