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Test Name:
Electrophoresis, Protein, with Immunofixation if Indicated, Serum
- SBMF No:
23296 - Performance Lab Name:
Manual Lab - Test Mnemonic:
IFE- - ABN:
Not required - CPT Code:
84155; 84165
If indicated, add 86334 - LOINC Code:
2885-2; 2862-1; 2865-4; 2868-8; 32730-4; 32731-2; 2874-6; 12851-2 - Test Includes:
Protein Electrophoresis
Immunofixation if Indicated - Also Known As:
Monoclonal Protein Study, Serum
Protein Electrophoresis, with Immunofixation if Indicated, Serum - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
3.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated (3 days) or frozen - Spec Stability:
7 days refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Hemolyzed sample - Methodology:
Zone Electrophoresis - Use:
Detect monoclonal or biclonal gammopathy; indicated when results of immunoelectrophoresis are equivocal; useful in recognition of a small monoclonal protein in the presence of a polyclonal increase in immunoglobulins - Reference Range:
Total Protein: 6.2-8.1 g/d
Albumin: 3.2-4.7 g/dL
Alpha 1: 0.09-0.23 g/dL
Alpha 2: 0.45-0.93 g/dL
Beta 1: 0.37-0.78 g/dL
Beta 2: 0.12-0.45 g/dL
Gamma: 0.52-1.37 g/dL - Day Run:
Mon-Sat - Time Run:
8:00 am - Time Reported:
2 days - Test Type:
PROTEIN
