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Test Name:
Electrophoresis, Protein, with Immunofixation, Urine, Random


  • SBMF No:
    23255
  • Performance Lab Name:
    Manual Lab
  • Test Mnemonic:
    U IFER
  • ABN:
    Not required
  • CPT Code:
    82664; 84156; 86335
  • LOINC Code:
    49276-9; 2888-6; 13992-3; 13990-7; 13993-1; 32736-1; 13995-6; 56768-5; 49299-1
  • Test Includes:
    Protein Electrophoresis
    Immunofixation if Indicated
  • Also Known As:
    Bence Jones Protein, Random Urine
    Monoclonal Protein Study, Random Urine
    Protein Electrophoresis with Immunofixation, Urine
  • Spec Type:
    Urine, random
  • Spec Container:
    Plastic container with tightly fitting lid
  • Pref Vol:
    20.0 mL aliquot from well-mixed collection
  • Min Vol:
    10.0 mL aliquot from well-mixed collection
  • Fasting:
    No
  • Spec Collect:
    Routine random urine collection
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Spec Remarks:
    Note: 24-hour urine is optimal specimen for testing
  • Methodology:
    Zone Electrophoresis
  • Use:
    Detect a monoclonal light chain in the urine of a patient with (or suspected to have) multiple myeloma, macroglobulinemia, amyloidosis, or related disorders
  • Clinical Significance:
    Immunofixation electrophoresis (IFE) is the most sensitive method used to detect, confirm, and then characterize the presence of a monoclonal gammopathy identified by serum protein electrophoresis in patients suspected of having a plasma cell dyscrasia (e.g., multiple myeloma, Waldenstrom's macroglobulinemia, monoclonal gammopathy of unknown significance [MGUS], light- or heavy-chain disease) or other lymphoproliferative process. IFE is particularly useful in identifying minor monoclonal proteins, the light chain component of monoclonal proteins, and unknown bands seen on serum protein electrophoresis.A monoclonal protein is found in the serum and/or urine of 99 percent of patients with multiple myeloma. Similar to the normal heavy chain distribution in serum, approximately 50-75 percent of monoclonal gammopathies are IgG, 20 percent are IgA, and less than one percent are IgD. IgM and
  • Reference Range:
    No proteins detected
  • Day Run:
    Mon-Fri
  • Time Run:
    8:00 am
  • Time Reported:
    2 days
  • Test Type:
    PROTEIN

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