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Test Name:
17-Hydroxyprogesterone, Serum or Plasma
- SBMF No:
23215 - Performance Lab Name:
Immunochemistry - Test Mnemonic:
17-OHP - ABN:
Not required - CPT Code:
83498 - LOINC Code:
1668-3 - Also Known As:
Hydroxyprogesterone
17-OHP
17a-OH Progesterone - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Lavender top (EDTA) or green top (lithium heparin) tube - Pref Vol:
0.5 mL - Min Vol:
0.2 mL - Fasting:
No - Patient Prep:
Patient must not have received isotopes during 24 hours period prior to sample collection - Spec Collect:
Routine venipuncture - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated (4 days) or frozen - Spec Stability:
4 days refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Methodology:
Radioimmunoassay (RIA) - Use:
17-OHP serum levels are primarily useful in the diagnosis of congenital adrenal hyperplasia (CAH) due to deficiency of 21-hydroxylase or 11 ß-hydroxylase. The assay has also been used to help identify the heterozygous state of 21- hydroxylase deficiency by testing before, and 30 and 60 minutes after, ACTH stimulation. - Clinical Significance:
17-hydroxyprogesterone (17-OHP, 4-pregren-17-ol-3, 20-dione) is a twenty-one-carbon steroid produced in the adrenals, gonads, and placenta. It is the metabolic product of progesterone and 17-hydroxypregnenolone and is a precursor to cortisol. A synthetic defect in 21-hydroxylase results in diminished cortisol synthesis, elevated ACTH, accumulation of 17-hydroxyprogesterone (particularly after the administration of ACTH), possible virilization due to increased adrenal androgen production, and possible salt wasting due to diminished production of aldosterone and deoxycorticosterone. 17-OHP serum levels exhibit a diurnal pattern similar to cortisol, and increase during the luteal phase of the menstrual cycle and during pregnancy.In the most common form of congenital adrenal hyperplasia, deficiency of the enzyme 21-hydroxylase blocks normal synthesis of cortisol, leading to a compensatory increase of ACTH secretion. This results in adrenal hyperplasia with increased levels of 17a-hydroxyprogesterone, DHEA and other adrenal steroids. - Reference Range:
Males: 0.42-3.5 ng/mL
Females:
- Follicular: 0.20-2.6 ng/mL
- Luteal: 0.28-4.0 ng/mL
- Oral contraceptives: 0.09-1.7 ng/mL
- Menopause, Hormone: 0.08-0.93 ng/mL
- Menopause, No Hormone: 0.11-1.2 ng/mL
Premature newborn: < 6.00 ng/mL
Newborns, 3rd day: < 0.08 ng/mL
Children: <0.65 ng/mL - Day Run:
Wed, Sat - Time Run:
8:00 am - Time Reported:
5:00 pm - Test Type:
HORMONE
