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Test Name:
Bilirubin, Direct
- SBMF No:
23203 - Performance Lab Name:
Automated Lab - Test Mnemonic:
BILI-DIR - ABN:
Not required - CPT Code:
82248 - LOINC Code:
1968-7 - Also Known As:
Direct Bilirubin - Also See:
23204 – Bilirubin, Total - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Alt Spec Type:
Plasma - Alt Spec Container:
Green top (lithium heparin) tube - Pref Vol:
1.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
Routine venipuncture
Label sample tube
Protect sample from light - Spec Process:
Serum sample tubes, clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to light-protected (amber) plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated - Spec Stability:
1 week refrigerated (2-8°C) and protected from light
6 months frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Methodology:
Colorimetric/Photometric - Use:
Used in the diagnosis of liver disease, in the detection of hemolytic anemia, and to evaluate degrees of jaundice - Clinical Significance:
Bilirubin is an organic compound formed by the reticuloendothelial system during the normal and abnormal destruction of red blood cells. Measurements of bilirubin are used in the diagnosis of liver disease, in the detection of hemolytic anemia, and to evaluate degrees of jaundice. - Reference Range:
0-2 weeks: 0.0-1.2 mg/dL
> 2 weeks: 0.0-0.3 mg/dL - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
CHEMISTRY