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Test Name:
Immunofixation, Urine


  • SBMF No:
    23064
  • Performance Lab Name:
    Manual Lab
  • Test Mnemonic:
    U IMMUNO
  • ABN:
    Not required
  • CPT Code:
    86335
  • LOINC Code:
    21482-5; 59465-5
  • Also Known As:
    Immunofix, Urine
    Immunofixation, Urine
  • Also See:
    23055 Electrophoresis, Protein, with Immunofixation, Urine, 24-Hour
    23255 Electrophoresis, Protein, with Immunofixation, Urine, Random
    23219 Kappa/Lambda Free Light Chains, Quantitative, with Ratio, Urine, 24-Hour
  • Spec Type:
    Urine, 24-hour
  • Spec Container:
    Plastic container with tightly fitting lid (no preservative)
  • Alt Spec Type:
    Urine, random
  • Alt Spec Container:
    Plastic container with tightly fitting lid
  • Pref Vol:
    10.0 mL aliquot from well-mixed 24-hour or random collection
  • Min Vol:
    5.0 mL aliquot from well-mixed 24-hour or random collection
  • Fasting:
    No
  • Spec Collect:
    To begin collection of timed specimen, have patient urinate, noting date and exact time
    Discard this first portion of urine, but document date and time of voiding on container label and test requisition as "start" of timed specimen
    Save all urine voided during designated time period, including portion voided at exact end of time period
    IMPORTANT: Refrigerate entire specimen (2-8°C) during and after collection to prevent bacterial growth
    Document date and time of final voiding on label and requisition as "end" of timed specimen
  • Spec Process:
    Upon completion of collection, mix entire specimen well and note 24-hour volume
    Pour 10.0 mL of urine into separate plastic, trace metal-free container
    Refrigerate urine aliquot and transport refrigerated
    Send urine aliquot with notation of 24-hour urine volume on aliquot label and on requisition
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Methodology:
    Immunofixation
  • Use:
    Detection of monoclonal proteins in urine
  • Reference Range:
    Interpretive report provided by pathologist
  • Day Run:
    Mon-Sat
  • Time Run:
    8:00 am
  • Time Reported:
    24 hours
  • Test Type:
    URINE