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Test Name:
Electrophoresis, Protein, with Immunofixation, Urine, 24-Hour


  • SBMF No:
    23055
  • Performance Lab Name:
    Manual Lab
  • Test Mnemonic:
    U IFE24
  • ABN:
    Not required
  • CPT Code:
    82664; 84156; 86335
  • Test Includes:
    Protein Electrophoresis
    Creatinine
    Immunofixation if Indicated
  • Also Known As:
    Bence Jones Protein, 24-Hour Urine
    Monoclonal Protein Study, 24-Hour Urine
    Protein Electrophoresis, with Immunofixation, 24-Hour Urine
  • Spec Type:
    Urine, 24-hour
  • Spec Container:
    Plastic container with tightly fitting lid (no preservative)
  • Pref Vol:
    20.0 mL aliquot from well-mixed 24-hour collection
    Note: Send urine aliquot with notation of entire 24-hour urine volume on aliquot label and on requisition
  • Min Vol:
    10.0 mL aliquot from well-mixed 24-hour collection
  • Fasting:
    No
  • Spec Collect:
    To begin collection of timed specimen, have patient urinate, noting date and exact time
    Discard this first portion of urine, but document date and time of voiding on container label and test requisition as "start" of timed specimen
    Save all urine voided during designated time period, including portion voided at exact end of time period
    IMPORTANT: Refrigerate entire specimen (2-8°C) during and after collection to prevent bacterial growth
    Document date and time of final voiding on label and requisition as "end" of timed specimen
  • Spec Process:
    Upon completion of the collection, mix the entire specimen well and measure the 24-hour volume
    Pour off 20.0 mL of urine into separate plastic tube(s)
    Refrigerate urine aliquot and transport refrigerated
    Send urine aliquot with notation of entire 24-hour urine volume on aliquot label and on requisition
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    1 week refrigerated (2-8°C)
  • Methodology:
    Zone Electrophoresis
  • Use:
    Detect a monoclonal light chain in the urine of a patient with (or suspected to have) multiple myeloma, macroglobulinemia, amyloidosis, or related disorders
  • Reference Range:
    Urine Protein: Less than 150 mg/24-Hour
    Albumin: 82.7 %
    Alpha 1: 2.1 %
    Alpha 2: 3.2 %
    Beta: 3.2 %
    Gamma: 8.6 %
  • Day Run:
    Mon-Fri
  • Time Run:
    8:00 am
  • Time Reported:
    2 days
  • Test Type:
    PROTEIN

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