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Test Name:
Electrophoresis, Protein, with Immunofixation, Serum
- SBMF No:
23051 - Performance Lab Name:
Manual Lab - Test Mnemonic:
PR EL/IFE - ABN:
Not required - CPT Code:
84155; 84165; 86334 - LOINC Code:
2865-4; 25700-6; 2868-8; 32730-4; 32731-2; 2874-6; 12851-2; 2885-2; 2862-1 - Test Includes:
Protein Electrophoresis
Immunofixation - Also Known As:
Monoclonal Protein Study, Serum
Protein Electrophoresis, with Immunofixation, Serum - Spec Type:
Serum - Spec Container:
Gold top (SST) or red top (serum) tube - Pref Vol:
3.0 mL - Min Vol:
0.5 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Clot 30 minutes
Promptly centrifuge 15 minutes
Immediately transfer serum to separate plastic tube
Properly centrifuged gel barrier tube sample does not require transfer of serum to separate tube - Spec Store Transport:
Refrigerated (3 days) or frozen - Spec Stability:
7 days refrigerated (2-8°C)
1 month frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Hemolyzed sample - Methodology:
Zone Electrophoresis - Use:
Screen for pathophysiologic states such as inflammation, protein loss, gammophathies, and other dysproteinemias - Reference Range:
Total Protein: 6.2-8.1 g/d
Albumin: 3.2-4.7 g/dL
Alpha 1: 0.09-0.23 g/dL
Alpha 2: 0.45-0.93 g/dL
Beta 1: 0.37-0.78 g/dL
Beta 2: 0.12-0.45 g/dL
Gamma: 0.52-1.37 g/dL - Day Run:
Mon-Sat - Time Run:
8:00 am - Time Reported:
2 days - Test Type:
PROTEIN