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Test Name:
Calcium, Ionized

• SBMF No:
  23026
• Performance Lab Name:
  Hospital Lab
• Test Mnemonic:
  CAI
• ABN:
  May be required – Medical Necessity
• CPT Code:
  82330
• LOINC Code:
  47596-2; 2746-6; 47598-8; 31208-2
• Test Includes:
  Ionized Calcium
  pH
  pH corrected Ionized Calcium
• Also Known As:
  iCa
  Ionized Calcium
• Also See:
  29039 Calcium, Serum or Plasma
• Spec Type:
  Whole blood
• Spec Container:
  Green top (lithium heparin) tube
• Alt Spec Type:
  Serum or plasma
• Alt Spec Container:
  Gold top (SST) tube or Light green top (PST) tube – CENTRIFUGED
• Pref Vol:
  2.0 mL
• Min Vol:
  2.0 mL heparin tube or 1.0 mL heparinized syringe
• Fasting:
  No
• Spec Collect:
  Routine venipuncture
• Spec Process:
  

  Do not open the tube or split the specimen
  Do not freeze
  Do not expose specimen to air at any time during collection or transport process

  Preferred specimen – tube of heparinized whole blood:
   - Mix specimen well by gently inverting end-to-end
   - Immediately place in container of ice slush (ice and water mixture)

  Alternate specimen – SST or PST tube:
   - For SST tube, allow blood to clot 30 minutes
   - Promptly centrifuge 15 minutes
   - Refrigerate entire tube of centrifuged blood

• Spec Store Transport:
  Whole Blood: In ice slush
  Serum or Plasma: Refrigerated
  Transport sample in original (un-opened) collection tube
• Spec Stability:
  Preferred heparinized whole blood tube sample: 4 hours in ice slush
  Alternate SST or PST tube sample: 4 days refrigerated (2-8°C)
• Spec Reject:
  Whole blood specimen not received in ice slush
  Whole blood specimen received more than 4 hours after collection
  Clotted whole blood
  Specimen exposed to air or frozen
• Methodology:
  Ion-selective Electrode (ISE)
• Use:
  Evaluate non-bound calcium, calcium metabolism, physiologically active calcium fraction, and hyperparathyroidism
• Reference Range:
  Ionized Calcium, pH corrected: 1.13-1.32 mEq/L
  pH (venous): 7.35-7.45
• Critical Low Value:
  0.77 mmol/L or less
• Critical High Value:
  1.59 mmol/L or greater
• Additional Test Info:
  
• Day Run:
  Sun-Sat (daily)
• Time Run:
  As received
• Time Reported:
  Routine: 1 hour
  STAT: 20 minutes
• Test Type:
  GAS-LYTE