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Test Name:
D-Dimer, Quantitative, Plasma
- SBMF No:
23017 - Performance Lab Name:
Hem-Coag - Test Mnemonic:
D-DIMER QN - ABN:
Not required - CPT Code:
85379 - LOINC Code:
48065-7 - Also Known As:
Dimer - Spec Type:
Platelet-poor plasma (PPP) - Spec Container:
Light blue top (3.2% buffered sodium citrate) tube - Pref Vol:
0.5 mL - Min Vol:
0.3 mL - Fasting:
No - Spec Collect:
- Routine venipuncture
- • NOTE: Traumatic puncture invalidates sample for coagulation studies and necessitates recollection
• Observe proper collection order for coagulation tests; collect tubes for coagulation studies prior to collecting tubes containing clot activators, additives, or anticoagulants
• Fill light blue top tube to stated tube volume
• If coagulation study is only test ordered, collect 1.0 to 2.0 mL blood into tube for discard, then collect light blue top coagulation study tube(s)
• If multiple coagulation studies are requested, submit additional samples for each coagulation test
• Immediately after collection, gently invert tube to mix
- Spec Process:
- Separate plasma from cells immediately
- • Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• NOTE: Buffy coat layer of sample must be avoided
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
• Immediately freeze PPP samples
- Spec Store Transport:
Frozen - Spec Stability:
4 hours room temperature (20-30°C)
2 weeks frozen (-20°C) – Do not use frost-free units that undergo repeated freeze/thaw cycles - Spec Reject:
Citrate tube underfilled
Sample clotted or hemolyzed
Plasma not frozen within 4 hours of collection - Methodology:
Quantitative Turbidimetric Assay - Use:
Aids in diagnosis of thromboembolic events: deep vein thrombosis (DVT), pulmonary embolism (PE), and disseminated intravascular coagulation (DIC)
This assay may be used in conjunction with a non-high clinical pre-test probability (PTP) assessment model to exclude deep-vein thrombosis (DVT) and pulmonary embolism (PE). - Clinical Significance:
Coagulation activation results in the cleavage of fibrinogen to fibrin. The resulting fibrin molecules spontaneously aggregate and are cross-linked by Factor XIII; this produced a fibrin clot. The activation of the fibrinolytic system results in the conversion of plasminogen into the active protease plasmin that cleaves fibrinogen and fibrin into the fragments D and E. Due to the cross-linkages between the D-domains in the fibrin clot, the action of plasmin releases fibrin degradation products with cross-linked D-domains. The smallest unit is the D-dimer. Detection of cross-linked degradation products, of D-dimers, thus is indicative of reactive fibrinolysis. - Reference Range:
Cutoff at 0.50 yields negative predictive
value of 100% for DVT and 99.5% for PE.Reference Range based on normal population
studies: 0.00-0.53 mg/L FEU. - Additional Test Info:
Results are reported in Fibrinogen Equivalent Units (FEU) - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
4 hours - Test Type:
COAGULATION
