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Test Name:
Fetal-Maternal Bleed, Qualitative, with Quantitation and RhoGAM if Indicated


  • SBMF No:
    22311
  • Performance Lab Name:
    Blood Bank Services
  • Test Mnemonic:
    RHIG PP
  • ABN:
    Not required
  • CPT Code:
    85461
  • LOINC Code:
    33900-2; 19066-0; 51784-7
  • Test Includes:
    Screen for Fetal-Maternal Bleed
    Rh Immune Globulin (RhoGAM), if Indicated
  • Also Known As:
    Post-partem RhoGAM Panel
  • Also See:
    36040 Fetal-Maternal Bleed, Quantitation, by Flow Cytometry
  • Spec Type:
    Whole blood
  • Spec Container:
    Lavender top (EDTA) tube
  • Pref Vol:
    7.0 mL
  • Min Vol:
    3.0 mL
  • Fasting:
    No
  • Spec Collect:
    Sample should be collected after delivery of all the products of conception and preferably as close to 1 hour after delivery as possible
    Routine venipuncture
  • Spec Process:
    Do not centrifuge
    Do not remove plasma from cells
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    48 hours refrigerated (2-8°C)
  • Methodology:
    Antigen-Antibody Reaction
    Qualitative Bleed Screen (agglutination)
  • Use:
    Used to determine the presence of (D) positive fetal cells in the maternal circulation
  • Clinical Significance:

    Every Rh-negative (weak D negative) mother who delivers or aborts a fetus after twelve weeks gestation must have a qualitative fetal-maternal bleed screen test by agglutination performed to determine the presence of (D) positive fetal cells in the maternal circulation.

    NOTE: If the newborn types Rh-positive (weak D), a quantitative fetal-maternal bleed test must be performed.

  • Reference Range:

    Negative

    Note:
    Rh Immune Globulin Dosing:
    If the qualitative fetal-maternal bleed screen test is negative, one (1) vial of Rh immune globulin is the suggested dose to be administered to the mother.
    If the qualitative fetal-maternal bleed screen test is positive, a quantitative fetal-maternal bleed test will be performed and Rh immune globulin dosing will be calculated based upon the quantitative test results.

  • Additional Test Info:
    Fetal-Maternal Bleed, Quantitation, by Flow Cytometry (SBMF #36040) is added and reported separately when indicated. Additional charges may apply.
    Vial(s) of RhoGAM are dispensed to the ordering client based upon test results
  • Day Run:
    Sat-Sun (daily)
  • Time Reported:
    If ordered stat, within 4 hours of receipt in lab
  • Test Type:
    BLOOD BANK

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