test directorySearch Results
Click here to search again.
Test Name:
Donor, West Nile Virus RNA Screen, by RT-PCR
- SBMF No:
22090 - Performance Lab Name:
Flow Cytometry/Molecular Pathology - Test Mnemonic:
NAT WN - ABN:
N/A - CPT Code:
N/A - LOINC Code:
41212-2 - Also Known As:
WNV NAT - Spec Type:
Plasma - Spec Container:
Lavender top (EDTA) tube
Other anticoagulants (CPD, CPDA1, CP2D, ACDA, and 4% Sodium Citrate) may be used at the discretion of the Department Manager - Pref Vol:
3.0 mL - Fasting:
No - Spec Collect:
Routine blood donor collection - Spec Stability:
For Condition 1, blood may be stored for up to 24 hours at 2-25°C followed by 72 hours at 2-25°C, prior to plasma separation. Following removal of K3 EDTA (and K2 EDTA) by centrifugation at 800–1600 xg for 20 minutes, plasma may be stored at 2–8°C for 7 days. Alternatively plasma may be stored at -20°C for up to 30 days.
For condition 2, blood may be stored for up to 72 hours at 2-25°C followed by 48 hours at 2-8°C, prior to plasma separtion. Following removal of K3 EDTA (and K2 EDTA) by centrifugation at 800–1600 xg for 20 minutes, plasma may be stored at 2–8°C for 7 days. Alternatively plasma may be stored at -20°C for up to 30 days.
K3 EDTA (and K2 EDTA) can be frozen and thawed a maximum of three (3) times.
- Spec Reject:
Frozen whole blood sample
Sample collected in Heparin anticoagulant - Methodology:
cobas® TaqScreen West Nile Virus Test - Use:
The cobas® TaqScreen West Nile Virus (WNV) Test is a qualitative in vitro test for the direct detection of West Nile Virus RNA in plasma. This product is intended for use as a donor-screening test for plasma samples from individual human donors, including donors of whole blood and blood components and other living donors. It is also intended for use to screen organ donors when specimens are obtained while the donor’s heart is still beating. This test is not intended for use on samples of cord blood.
Plasma from all donors may be screened as individual samples. For donations of whole blood and blood components, plasma may be tested in pools comprised of not more than six equal aliquots of individual specimens.
This assay is not intended for use as an aid in diagnosis.
- Clinical Significance:
West Nile Virus (WNV) is a member of the Flaviviridiae family, the genus Flavivirus and the Japanese encephalitis virus serocomplex. Viruses in this complex are arboviruses with the potential to cause meningitis, encephalitis and meningoencephalitis. Other members of the Japanese encephalitis group include Japanese encephalitis virus, Murray Valley encephalitis virus, Kunjin (now known to be variant of WNV) and St. Louis encephalitis virus; the latter caused an epidemic of encephalitis in the United States in the mid-1970’s.
The cobas® TaqScreen West Nile Virus Test is a qualitative test that enables the screening and detection of WNV RNA in infected pooled and individual specimen donations. The cobas® TaqScreen West Nile Virus Test uses a generic nucleic acid preparation technique on the COBAS® AmpliPrep Instrument. WNV RNA is then detected by automated, real time PCR amplification on the COBAS® TaqMan® Analyzer. The test incorporates an Internal Control for monitoring test performance in each individual test as well as the AmpErase enzyme to reduce potential contamination by previously amplified material (amplicon).
- Reference Range:
Negative - Test Type:
BLOOD BANK
