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Test Name:
Direct Antiglobulin Test
- SBMF No:
22057 - Performance Lab Name:
Blood Bank Services - Test Mnemonic:
DAT - ABN:
Not required - CPT Code:
86880 - LOINC Code:
1007-4 - Also Known As:
DAT
Direct Coombs (Anti-Human Globulin) - Spec Type:
Whole blood - Spec Container:
Lavender top (EDTA) tube - Pref Vol:
7.0 mL - Min Vol:
1.0 mL - Fasting:
No - Spec Collect:
Routine venipuncture - Spec Process:
Do not centrifuge
Do not remove plasma from cells - Spec Store Transport:
Refrigerated - Spec Stability:
24 hours refrigerated (2-8°C) - Methodology:
Antigen-Antibody Reaction - Use:
It is commonly used in the diagnosis of hemolytic disease of the newborn, autoimmune hemolytic anemia, drug-induced red cell sensitization, and for investigation of transfusion reactions - Clinical Significance:
The direct antiglobulin (Coombs) test is a one-stage test for the detection of in vivo red cell sensitization. It is commonly used in the diagnosis of hemolytic disease of the newborn, autoimmune hemolytic anemia, drug-induced red cell sensitization, and for investigation of transfusion reactions. Depending on the type of specimen used, a positive reaction indicates the presence of antibodies and/or complement attached to the cell surface. - Reference Range:
Negative - Day Run:
Mon-Fri - Time Run:
As received - Time Reported:
Within 8 hours - Test Type:
BLOOD BANK
