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Test Name:
Rh Blood Type


  • SBMF No:
    22004
  • Performance Lab Name:
    Blood Bank Services
  • Test Mnemonic:
    RH
  • ABN:
    Not required
  • CPT Code:
    86901
  • LOINC Code:
    10331-7
  • Also Known As:
    Blood Type
  • Spec Type:
    Whole blood
  • Spec Container:
    Lavender top (EDTA) tube
  • Alt Spec Type:
    Cells and serum
  • Alt Spec Container:
    Red top (serum) tube
  • Pref Vol:
    6.0 mL
  • Min Vol:
    1.0 mL
  • Fasting:
    No
  • Spec Collect:
    Do not use gel-barrier tubes for specimen collection
    Routine venipuncture
  • Spec Process:
    Do not centrifuge
    Do not remove serum or plasma from cells
  • Spec Store Transport:
    Refrigerated
  • Spec Stability:
    24 hours room temperature (20-30°C)
    2 weeks refrigerated (2-8°C)
  • Spec Reject:
    Hemolyzed sample
  • Methodology:
    Antigen-Antibody Reaction
  • Use:
    Determine blood type prior to transfusion, on expectant mothers during pregnancy, and on newborn blood samples
    Work-up candidacy for Rho(D) immune globulin to detect possible ABO hemolytic disease of the newborn
    As part of "Type and Screen" to protect mother or surgical patient in case of obstetric or other hemorrhage
  • Clinical Significance:
    Tests may be performed using a human source of anti-serum or with a monoclonal blend anti-serum.Because the D antigen is clinically significant and highly antigenic, the determination of its presence or absence in donor or recipient processing and in routine blood typing is essential.Reactivity of the D antigen may vary from one person to another. The reaction in vitro may be so weak that antiglobulin testing may be required in order to detect it. Cells that are positive only after antiglobulin testing are called Rh-positive, weak D (formerly referred to as Du variant).
  • Day Run:
    Mon-Fri
  • Time Run:
    10:00 pm
  • Time Reported:
    10:00 am next day
  • Test Type:
    BLOOD BANK