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Test Name:
Static-Cidal Level, Blood
- SBMF No:
21141 - Performance Lab Name:
Microbiology - Test Mnemonic:
STATIC - ABN:
Not required - CPT Code:
87197 - LOINC Code:
- Test Includes:
Titration of serum bacteriostatic and bactericidal activity against patient isolate - Also Known As:
Antibacterial Activity
Bacterial Inhibitory Level
Maximum Bactericidal Dilution (MBD)
MBD
Schlichter Test;
Serum Bactericidal Level
Serum Cidal Test - Spec Type:
Serum - Spec Container:
Red top (serum) tube in a container of ice slush (ice and water mixture) - Min Vol:
2.0 mL - Fasting:
No - Spec Collect:
Do not use gel-barrier tubes for specimen collection
Cleanse skin over site of venipuncture with 70% alcohol and tincture of iodine
Allow tincture of iodine to dry 1 minute prior to venipuncture
Requires 2 samples:
1) Trough (predose) collected 30 minutes before antibiotic dosage
2) Peak (postdose) collected:
- 30-45 minutes after completion of 15-30 minutes IV infusion -or-
- 60 minutes after IM injection dose -or-
- 90 minutes after oral dose
Promptly place blood tubes in container of ice slush (ice and water mixture) - Spec Process:
Chill specimen in ice slush (ice and water mixture) during clotting
Clot at least 30 minutes with tube remaining in ice slush (ice and water mixture)
Note: chilling may prolong clotting time
Remove from ice slush and immediately centrifuge 15 minutes
Use aseptic technique to promptly transfer serum from each specimen to separate sterile tubes - Spec Store Transport:
Refrigerate or chill in ice slush (ice and water mixture)
If specimen will not be transported on day collected, freeze and transport frozen - Spec Reject:
Sample not iced upon collection
Serum not refrigerated or frozen
Mistimed sample collection - Methodology:
Serial Dilution - Use:
Titration of serum bacteriostatic and bactericidal activity against patient isolate used to determine the extent to which serum or fluid from a patient on antibiotic therapy could be diluted and still inhibit and destroy the growth of an infecting organism isolated from that patient - Clinical Significance:
The patient specimen is serially diluted and then inoculated with a known concentration of infecting organism isolated from the same patient. After incubation, the inhibitory titer is determined as the highest dilution showing no growth of the organism. The cidal level is then determined from subcultures of the inhibitory dilutions. - Reference Range:
No universally accepted standard for interpretation - Day Run:
Sun-Sat (daily) - Time Run:
As received - Time Reported:
Static: 24 hours
Cidal: 48 hours - Test Type:
MICROBIOLOGY
