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Test Name:
Vaginosis, Bacterial, Gram-Stain


  • SBMF No:
    21086
  • Performance Lab Name:
    Microbiology
  • Test Mnemonic:
    BV SMEAR
  • ABN:
    Not required
  • CPT Code:
    87205
  • LOINC Code:
    43391-2
  • Also Known As:
    Bacterial Vaginosis Smear
    BV Smear
  • Also See:
    25133 – Wet Mount for Trichomonas, Yeast, and Clue Cells
  • Spec Type:
    Vaginal smear prepared at time of pelvic exam
  • Spec Container:
    Glass slide
  • Alt Spec Type:
    Swab
  • Pref Vol:
    Two (2) smears
  • Min Vol:
    One (1) swab
  • Fasting:
    No
  • Spec Collect:
    Collected by physician
  • Spec Store Transport:
    Specimen and/or labeled slide - room temperature
  • Spec Stability:
    Room temperature (20-30°C)
  • Methodology:
    Microscopic Examination
  • Use:
    Definitive laboratory method for diagnosis of bacterial vaginosis (BV)
  • Clinical Significance:
    Bacterial vaginosis (BV) is a clinical syndrome characterized by an abnormal vaginal discharge in women of childbearing years. The syndrome has been associated with preterm birth, miscarriage, amniotic infections, and postpartum endometritus. The microbiota of the vagina shifts from predominantly lactobacilli to a mixture of Gardnerella vaginalis, Prevotella spp., Mobiluncus spp., and often other anaerobes and Mycoplasma hominis. The Gram stain with scoring of relative amounts of microbial morphotypes is the definitive laboratory method for diagnosis.
    If observed, the presence of yeast or trichomonads will be reported.
    The diagnosis of vaginal flora alterations in peri- and post-menopausal women by this method is inadequate, since the absence or reduction of lactobacilli in this age group is non-pathological. The diagnosis of purulent vaginitis is associated with the presence of Trichomonas vaginalis
  • Reference Range:
    Nugent Score: 0-3 = Normal
    Nugent Score: 4-6 = Intermediate, repeat test later
    Nugent Score: 7-10 = Bacterial vaginosis
  • Day Run:
    Sun-Sat (daily)
  • Time Run:
    Day shift, Second shift, Third shift
  • Time Reported:
    4:00 am, 8:00 am, 4:00 pm, 11:30 pm
  • Test Type:
    MICROBIOLOGY