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Test Name:
Helicobacter pylori Rapid Urease Test, Mucosal Biopsy


  • SBMF No:
    21040
  • Performance Lab Name:
    Microbiology
  • Test Mnemonic:
    H PYLORI
  • ABN:
    Not required
  • CPT Code:
    87081
  • LOINC Code:
    32637-1
  • Also Known As:
    H. pylori, Urease, Tissue
    RUT
    CLO Test
    Mucosal Biopsy H. pylori
  • Spec Type:
    Gastric biopsy tissue sample
  • Spec Container:
    "Selective Rapid Urea" media
  • Pref Vol:
    3 mm diameter
  • Min Vol:
    2 mm diameter
  • Fasting:
    No
  • Spec Collect:
    Allow "Selective Rapid Urea" media to warm to room temperature
    Collected by physician during surgical procedure
    Place tissue biopsy, with a sterile 19-gauge needle, into the "Selective Rapid Urea" media
    Ensure that tissue is completely immersed in order to provide maximum media contact
    Label "Selective Rapid Urea" vial with patient name, date, and time of media inoculation
  • Spec Process:
    Maintain in closed container of "Selective Rapid Urea" media at room temperature
  • Spec Store Transport:
    Room temperature
  • Spec Stability:
    Room temperature (20-30°C)
  • Methodology:
    Rapid Urease
  • Use:
    Detect presence of H. pylori in tissue sample
  • Clinical Significance:
    Helicobacter pylori has been shown to cause active chronic gastritis and has been implicated as a primary etiological factor in duodenal ulcer disease, gastric ulcer, and non-ulcer dyspepsia. By causing chronic inflammation, H. pylori may weaken the mucosal defenses and allow acid and pepsin to disrupt the epithelium. H. pylori produces large amounts of urease enzyme. Although H. pylori can be detected with histology or culture of gastric tissue, simple tests for the presence of urease enables more rapid and convenient diagnosis.
  • Reference Range:
    Negative for H. pylori
  • Additional Test Info:
    Test Limitations:
    Patients should not have taken antibiotics or bismuth salts for at least 3 weeks prior to endoscopy
    False-positives could occur in patients who have achlorhydria (as in pernicious anemia, previous gastric surgery or patients who have recently taken antacids)
    False-positives may also occur if other urea-splitting organisms are present in the specimen
  • Day Run:
    Sun-Sat (daily)
  • Time Run:
    Second Shift
  • Time Reported:
    Within 24 hours
  • Test Type:
    MICROBIOLOGY

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